. Author manuscript; available in PMC: 2008 Oct 3.

Published in final edited form as: Mol Reprod Dev. 2007 Sep;74(9):1228–1235. doi: 10.1002/mrd.20682

Table 5.

Effects of variety of osmolalities in PZM3 with SAA on the development of porcine IVF embryo

Osmolality of the first 72 h culture	Osmolalty after 72 h culture	No. of oocytes	Cleaved (Mean %± SEM)	Blastocyst (Mean %± SEM)	Nuclei No. of (Mean ± SEM)
270±2	250±2	98	80 (81.6±2.8)	29 (29.6±5.3)^ab	38.2±2.5^abc
270±2	270±2	100	83 (83.0±3.8)	21 (21.0±4.1)^a	41.9±7.3^abc
290±2	250±2	101	85 (84.2±7.1)	26 (25.7±5.2)^ab	48.2±11.2^a
290±2	270±2	97	84 (86.6±6.6)	28 (28.9±6.6)^ab	37.3±1.7^abc
300±2	250±2	100	87 (87.0±1.8)	29 (29.0±3.4)^ab	34.3±3.7^bc
300±2	270±2	98	85 (86.7±2.1)	27 (27.6±3.1)^ab	40.0±3.3^abc
330±2	250±2	108	92 (85.2±1.3)	36 (33.3±3.4)^b	33.5±2.3^bc
330±2	270±2	110	95 (86.4±2.6)	35 (31.8±1.7)^ab	36.3±4.7^bc
360±2	250±2	106	87 (82.1±1.9)	33 (31.1±4.5)^ab	32.9±2.7^bc
360±2	270±2	107	86 (80.4±2.2)	28 (26.2±3.0)^ab	30.0±3.7^c
288±2	288±2	104	81 (77.9±4.7)	22 (21.2±2.2)^a	46.6±8.4^ab

Values with different superscripts within the same column differ (p<0.05). Data represents four replicates.