Figure 1.

ARNO regulates cytoskeletal reorganization in HeLa cells treated with PMA. HeLa cells on coverslips were transiently transfected with empty vector (A and C) or vector encoding epitope-tagged ARNO (B and D). Cells were treated for 30 min without (A and B) or with 1 μM PMA (C and D), and then fixed in 2% formaldehyde. ARNO was detected by labeling with the monoclonal antibody 9E10 (anti-myc epitope) followed by Cy2-labeled donkey anti-mouse IgG (left panels). Cells were costained with rhodamine-labeled phalloidin to visualize filamentous actin (right panels).