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A Sec7 domain mutant is incapable of catalyzing nucleotide exchange on ARF6. Myristoylated ARF6 (1 μM) was incubated at 37°C in the absence (open circles) or presence of either 100 nM recombinant His6-ARNO (closed circles) or His6-E156K (open triangles) as described in MATERIALS AND METHODS. Data are the average of two simultaneous determinations and are representative of three separate experiments.
