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Catalytically inactive ARNO does not support cytoskeletal rearrangements in response to PMA treatment. HeLa cells transfected with plasmid encoding myc-tagged wild-type ARNO (A) or ARNO/E156K (B and C) were incubated in the presence (A and C) or absence (B) of PMA, fixed, and labeled for immunofluorescence as in Figure 1. Note the presence of intact stress fibers in panel B.
