Table 2.
Characterization of several mitochondrial and mtDNA features in transmitochondrial cybrids
| Cell line | mtDNA | mtDNA levels (mean ± SD)a | Mitochondrial content (CS/LDH) (mean ± SD) | mtDNA/Mitochondrial content | mtDNA repopulation rate (% rec/d)b | Repopulation rate/wild-type ratec |
|---|---|---|---|---|---|---|
| 143B | Wild-type | 2.35 ± 0.12 | 0.22 ± 0.03 | 10.7 | 1.7 | 1.4 |
| 143B/206 | ρ° | 0 | 0.36 ± 0.06 | NA | NA | NA |
| W20 | Wild-type | 2.49 ± 0.26 | 0.33 ± 0.07 | 7.6 | 0.8 | 0.6 |
| W72 | tRNAAsn G5703A | 3.27 ± 0.69 | 0.35 ± 0.09 | 9.3 | 2.3 | 1.8 |
| Δ16.10.40 | 100% 7.5-kb deletion | 4.17 ± 0.15 | 0.31 ± 0.04 | 13.5 | 6.0 | 4.8 |
| ΔBH10.5.9 | 88% 4.9-kb deletion | 3.43 ± 0.31 | 0.37 ± 0.11 | 9.3 | 9.4 | 7.5 |
| ΔHH6.1 | 89% 7.5-kb deletion | 6.27 ± 1.20 | 0.28 ± 0.03 | 22.4 | 9.2 | 7.4 |
| HC14 | Common chimp | NA | 0.33 ± 0.04 | NA | 2.0 | 1.6 |
| HP4 | Pigmy chimp | NA | 0.40 ± 0.02 | NA | 0.8 | 0.6 |
| HG13 | Gorilla | NA | 0.31 ± 0.02 | NA | 2.0 | 1.6 |
All cell lines have the same nuclear background (143B/TK−). NA, not applicable.
a
Ratio of mtDNA hybridization (ND1 region) and 18S rDNA (arbitrary units). The levels observed in nonhuman apes cannot be directly compared with the human cell lines, because the hybridized region (ND1) has ∼84% identity with the ape mtDNAs.
b
mtDNA repopulation rates were calculated for the initial 7 d after EtBr removal (see Figure 5).
c
The wild-type rate was assumed to be the average between 143B and W20 values.