Figure 1.

Main modes of three-dimensional visualization used in this work, exemplified by a prometaphase cell. (A) Projection mode with maximum intensity applied to the whole volume. (B) The initial volume was cut and sectioned chromosomes appear on the faces of the cube. This procedure allows us to examine the chromosomes in more detail within the volume. (C) Volumic visualization giving rise to a very informative view and showing the high complexity of the chromosomes (blue). (D) Same procedure, but chromosomes are partially transparent; this allows us to position UBF immunolabeled structures (yellow) within the set of chromosomes. Bars, 2 μm.