Figure 7.

Volumic visualization of an entire anaphase cell. (A) Chromosomes (blue) protruding at the periphery of the two sets are evidenced. (B) A stereo pair of the polar view shows the presence of a regular circular depression. The fusing chromosomes appear as several large masses, limited with radial furrows. (C) Median view of a set showing the presence of radially disposed and unfused chromosomes. (D) The transversal sectioning of this set shows the concave polar face with the depression, the concave median face, and the presence of NORs (yellow) within the central part of the set. (E and F) By rendering DNA transparent, the NORs are clearly localized within the central part of the set. They are disposed as a ring at the bottom of the depression (E). Bars, 2 μm.