Figure 1.
LCH-7 recognizes an antigen in the Golgi complex of pancreas acinar and epithelial NRK cells. Rat pancreas sections and NRK cells were fixed and processed for double-label indirect immunofluorescence as described. Samples were incubated with the mAb LCH-7 (A and C) and the Golgi marker rabbit anti-Man II (B and D). Colocalization of LCH-7 and Man II can be seen in the Golgi of pancreas acinar and NRK52E cells (A–D). A limited amount of staining for Man II is also observed on the plasma membrane of the acinar cells (B). Bar in D, ∼10 μm.