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. 1998 Nov;9(11):3161–3177. doi: 10.1091/mbc.9.11.3161

Figure 3.

Figure 3

Protein localization during kidney development. Kidney sections from neonatal CD1 mice were subjected to double immunofluorescence with polyclonal antibodies to Na/K-ATPase, ankyrin-3, fodrin, E-cadherin, and a monoclonal antibody to cytokeratin 8. (A, B) Field from neonatal kidney that was subjected to double immunofluorescence with anti-Na/K-ATPase and anti-cytokeratin 8 antibodies. Na/K-ATPase was detected using a rhodamine-labeled secondary antibody, and cytokeratin 8 was detected using a fluorescein-labeled secondary antibody. (A) Na/K-ATPase staining alone; (B) double exposure for Na/K-ATPase and cytokeratin 8 staining. (C, D) Field from neonatal kidney that was subjected to double immunofluorescence with antibodies to ankyrin-3 and cytokeratin 8. Ankyrin-3 was detected using a rhodamine-labeled secondary antibody, and cytokeratin 8 was detected using a fluorescein-labeled secondary antibody. Ankyrin-3 staining is shown alone (C) or as a double exposure with cytokeratin 8 staining (D). Insets in C and D show a comma-shaped body from a different field immediately beneath a branch of the ureteric bud. The ureteric bud can be identified by its prominent cytokeratin 8 staining in D. (E, F) Field from neonatal kidney that was subjected to double immunofluorescence with anti-fodrin and anti-cytokeratin 8 antibodies. Fodrin was detected using a rhodamine-labeled secondary antibody, and cytokeratin 8 was detected using a fluorescein-labeled secondary antibody. (E) Fodrin staining alone; (F) double exposure for fodrin and cytokeratin 8 staining. (G, H) Field from neonatal kidney that was subjected to double immunofluorescence with antibodies to E-cadherin and cytokeratin 8. E-cadherin was detected using a rhodamine-labeled secondary antibody, and cytokeratin 8 was detected using a fluorescein-labeled secondary antibody. E-cadherin staining is shown alone (G) or as a double exposure with cytokeratin 8 staining (H). Asterisks mark comma-shaped bodies that are found immediately below them. Arrows in A and B point to other early nephrogenic structures that are identified by weak basal and apical cytokeratin 8 staining. All fields are shown at the same magnification. Bar, 50 μm.