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. 1999 Oct;10(10):3373–3387. doi: 10.1091/mbc.10.10.3373

Figure 5.

Figure 5

Migration of U251 transfectants. Aggregates of U251NG2.51 (a–c), U251NG2/t3.4 (d–f), U251NG2/L1.7 (g–i), and rat mesangial cells (mesang, j–l) were plated on surfaces coated with mAb β1, mAb D120, mAb N143, or PLL. Cultures were photographed after 16 h to allow assessment of cell migration out of the aggregates. All three U251 cell populations exhibit good migration on mAb β1 and very poor migration on PLL. Differences in migration can be seen on mAb D120, where wild-type transfectants migrate very well (b), NG2/L1 transfectants migrate poorly (h), and NG2/t3 transfectants exhibit an intermediate level of migration (e). Mesangial cells migrate well on NG2 mAbs N143 (j) and D120 (k) but very poorly on PLL (l). Bar in c, 200 μm.