Table 1.
Comparison of NG2 expression
| Cells | NG2 expression (%) |
|---|---|
| Rat-1 | 100 |
| Mesangial | 87 |
| B28NG2.6 | 155 |
| B28NG2.8 | 172 |
| B28NG2/CNTN.M | 121 |
| B28NG2/CNTN.D | 88 |
| B28NG2/t3.6 | 148 |
| B28NG2/t3.8 | 167 |
| U251NG2.51 | 265 |
| U251NG2.35 | 281 |
| U251NG2/CNTN.40 | 187 |
| U251NG2/CNTN.10 | 203 |
| U251NG2/L1.7 | 273 |
| U251NG2/L1.3 | 302 |
| U251NG2/t3.4 | 288 |
| U251NG2/t3.18 | 244 |
NP40 extracts of 5 × 105 cells were treated with chondroitinase ABC, fractionated by SDS-PAGE, and analyzed by immunoblotting with affinity-purified rabbit antibody against NG2 (similar to the examples shown in Figure 2C). After developing the immunoblots with an ECL kit, we used densitometry to compare quantitatively the intensities of the NG2 bands from all of the cell lines. The quantity of NG2 found in rat-1 fibroblasts was arbitrarily assigned a value of 100%, and all other values are expressed in relation to this standard.