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. Author manuscript; available in PMC: 2008 Dec 15.
Published in final edited form as: Anal Chem. 2007 Nov 10;79(24):9364–9371. doi: 10.1021/ac071574q

Figure 4.

Figure 4

Focusing of IEF markers in the cascaded design. Focusing at the exit of the first stage (a) shows a fluorescent profile (solid line) similar to our earlier work.10 Here, a Gaussian peak (dotted line) is fitted to the peak of the pI 5.1 marker, yielding a steady-state resolution of 5.9 after 6.8 seconds of focusing. Intensities at the exit of the second stages (b) show greater resolution and concentration of the markers after an additional 20.4s of focusing. A Gaussian fit to the pI 5.1 marker calculates a resolution of 10.7, consistent with theory.