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. 1999 Oct;10(10):3389–3400. doi: 10.1091/mbc.10.10.3389

Figure 4.

Figure 4

Skn7 is not part of the core MBF. MBF was analyzed by gel retardation with a radioactively labeled 3xMCB probe (Lowndes et al., 1991). (A) Deletion of SKN7 does not affect MBF. Increasing amounts of crude extract were assayed from the strains shown. Note that the mbp1 mutation used is a point mutation (an allele of bor2) rather than a deletion. (B) Antibodies to Skn7 do not supershift MBF. Lane 1, free probe; lanes 2–10, wild-type (W303) crude extract; lane 2, no competitor; lane 3, competition with a 100-fold excess of the cold probe; lanes 4–6, preimmune serum (1:100, 1:200, 1:500); lanes 7–9, anti-Skn7 antibodies (1:100, 1:200, 1:500); lane 10, anti-Swi6 antibodies (1:200); lane 11, W303 skn7Δ crude extracts with anti-Swi6 antibodies (1:200).