Figure 1.

Modulation of CE cell shape. (A–F) Phase contrast views of CE cells cultured on FN-coated substrates; (G–I) schematic diagrams illustrating designs used to create micropatterned adhesive islands used for studies shown in D–F, respectively (all at same magnification). (A) High-density FN (3 μg/cm²); (B) low-density FN (25 ng/cm²); (C) high-density FN in the presence of cyto D (1 μg/ml); (D and G) 80-μm-square adhesive islands; (E and H) 30-μm-square adhesive islands; (F and I) 5-μm circular adhesive islands. Note that CE cells extended over multiple small dotlike adhesive islands separated by 10-μm-wide uncoated regions, whereas they remained restricted to single larger-size islands that are separated by larger nonadhesive boundary regions (100 μm wide). Shaded areas in diagrams represent adhesive regions coated with high-density FN.