Figure 3.

Control of cell shape, cytoskeletal organization, nuclear size, and pRB hyperphosphorylation on micropatterned adhesive islands. Shown are fluorescence views of CE cells cultured on square adhesive islands 30, 60, or 80 μm wide and stained for F-actin using FITC-phalloidin, nuclei using the DNA binding dye DAPI, or nuclear-associated (hypophosphorylated) pRb using a monoclonal antibody. A rare occurrence can be seen at the bottom right, in which three cells are adherent to a single 80-μm-wide island; note that these three smaller cells retain the hypophosphorylated form of pRB in their nuclei, whereas the single cell that is fully spread on the adjacent island at the left lacks nuclear pRB staining and hence, underwent pRb hyperphosphorylation.