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Mapping the shape-sensitive restriction point. (A) Western blots of total cell lysates from synchronized CE cells plated on high vs. low FN probed with antibodies against total p44/p42MAPK and phosphorylated (activated) p44/p42MAPK. (B) Steady-state mRNA levels of the G1-associated genes, E2F-1, and cyclins D1, D3, and E compared with β2-microglobulin (β2 M) in CE cells grown for the indicated times on high vs. low FN, as determined using RT-PCR.
