Figure 6.

Effects of varying FN density and cell spreading on cell cycle–associated proteins. (A) Western blots of total cell lysates from cells cultured for indicated times on high vs. low FN probed with antibodies against cdks 2 and 4 and cyclins D1, D3, and E, p21^Cip1(p21), or p27^Kip1 (p27). (B) Western blot comparing levels of cdk2 and p27^Kip1 (p27) in total cell lysates of synchronized CE cells cultured on low FN, high FN, micropatterned squares 30 μm wide (Figure 1, E and H), micropatterned dots 5 μm in diameter (Figure 1 F and I), or on high FN in the presence of cyto D (1 μg/ml) added at the indicated times. All lysates were obtained 18 h after plating.