Figure 7.

Role of the actin cytoskeleton and cytoskeletal tension in G1 progression. (A) Cyto D (1 μg/ml) was added at the indicated times to cultures of synchronized CE cells plated on high FN. Cumulative BrdU incorporation into DNA (black bars) and pRb hyperphosphorylation (stippled bars) were measured at 30 and 20 h, respectively, as described in Figure 2, and shown as relative values compared with the untreated control cultures. Error bars indicate SD. (B) Lat B (0.1 μg/ml), cyto D (1 μg/ml), and BDM (20 mM), as indicated in the inset legend, were added at the indicated times and BrdU incorporation determined as in A in parallel studies.