Figure 5.

Replacement of the extracellular domain of VEGFR-2 with the extracellular domain of human CSF receptor-1 does not alter the behavior of VEGFR-2 when expressed in PAE cells. PAE cells expressing an empty vector (X²) or chimeric VEGFR-2 (CK) were cultured in the confluent or sparse condition, serum starved overnight, and stimulated with CSF (40 ng/ml) for 5 min. The cells were lysed and immunoprecipitated with an anti-VEGFR-2 antibody and imunoblotted with an anti-phosphotyrosine (pY) antibody (A). The same membrane was reprobed with an anti-VEGFR-2 antibody (B).