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. 1998 Nov;9(11):3195–3209. doi: 10.1091/mbc.9.11.3195

Figure 3.

Figure 3

Neuregulin effects on different epithelial cell lines. Cells of squamous carcinoma A431 (A and B), prostate carcinoma DU 145 (C and D) and ovarian carcinoma IGROV (E and F) were plated at low density and maintained in serum-containing medium for 3 d. After formation of cell colonies (A, C, and E), the medium was changed to serum-free medium, and neuregulin (20 ng/ml) was added for an additional 6 h. The majority of colonies in the cultures of A431 and DU 145 cells were transformed into ring-shaped structures (B and D). Colonies of IGROV cells tended to undergo scattering but first formed some ring-shaped multicellular complexes (F). Note spreading of cell colonies and stimulation of lamellipodial activity at the outer front of the multicellular rings after neuregulin treatment (B, D, and F). Bar, 30 μm.