Figure 9.

Activation of c-Met receptor by HGF/SF and neuregulin treatment. Control N87 cells (lane 1) and the same cells activated with 10 ng/ml NDF-β4 (lanes 2–7) or with a 1:2 dilution of HGF-conditioned medium (lanes 8–13) for 1 min (lanes 2 and 8), 2 min (lanes 3 and 9), 5 min (lanes 4 and 10), 10 min (lanes 5 and 11), 30 min (lanes 6 and 12), and 60 min (lanes 7 and 13) were lysed, immunoprecipitated with anti-c-Met antibodies, and immunoblotted with anti-phosphotyrosine (A) or anti-c-Met antibodies (B). Note that NDF-β4 stimulation induced only a weak and transient increase in tyrosine phosphorylation of c-Met receptor compared with HGF/SF treatment of the same cells.