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. Author manuscript; available in PMC: 2008 Oct 3.
Published in final edited form as: Biochemistry. 2005 Dec 20;44(50):16776–16784. doi: 10.1021/bi0515568

FIGURE 3.

FIGURE 3

Apparent affinities of wild-type and mutant forms of IDH for the COX2 mRNA transcript. Data from SPR measurements conducted with buffer containing no ligand (black bars) or containing 2 mM DL-isocitrate and 50 μM AMP (white bars) were used to calculate apparent Kd values of immobilized forms of IDH for the COX2 UTR transcript as described under Experimental Procedures and in the legend for Figure 2. Values were obtained from duplicate samples in two or three independent experiments.