Indirect immunofluorescent localization of the Hmg1:β-gal fusions or Kar2p is shown in A, C, E, and G. Localization of the nucleus in the same cells is shown by DAPI staining in B, D, F, and H. (A and B) Localization of elevated levels of Hmg1:β-gal was determined in strain RWY 943 by using anti-β-gal antibody. (C and D) Localization of Kar2p in strain RWY 943 is shown for comparison. (E and F) Localization of elevated levels of Hmg1:β-gal 20 was determined in strain RWY 944 by using the same anti-β-gal antibody. (G and H) Localization of Kar2p in strain RWY 944 is shown for comparison. Bar, 5 μm.