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Localization of the truncated Hmg1:HA protein by indirect immunofluorescence. Indirect immunofluorescent localization of the truncated Hmg1:HA protein or Kar2p is shown in A and C. Localization of the nucleus in the same cells is shown by DAPI staining in B and D. (A and B) Localization of elevated levels of the truncated Hmg1:HA protein in strain RWY 614 was detected by using 12CA5 anti-HA antibody. (C and D) Localization of Kar2p in strain RWY 614 is shown for comparison. Bar, 5 μm.
