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. 1999 Oct;10(10):3409–3423. doi: 10.1091/mbc.10.10.3409

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Copyright © 1999, The American Society for Cell Biology

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Localization of the Hmg1^mem:GFP fusion and the Hmg1^mem:Hmg1^525–987:GFP fusion by indirect immunofluorescence. Indirect immunofluorescent localization of the GFP fusion proteins or Kar2p in shown in A, C, E, and G. Localization of the nucleus in the same cells is shown by DAPI staining in B, D, F, and H. (A and B) Localization of elevated levels of the Hmg1^mem:GFP protein in strain RWY 626 was determined by using a GFP antibody. (C and D) Localization of Kar2p in strain RWY 626 is shown for comparison. (E and F) Localization of elevated levels of the Hmg1^mem:Hmg1^525–987:GFP protein was determined in cells of strain RWY 621 using a GFP antibody. (G and H) Localization of Kar2p in strain RWY 621 is shown for comparison. Bar, 5 μm.