Table 3.
Expression of the soluble catalytic domain interfered with the production of karmellae without lowering the level of Hmg1p holoprotein
| Strain | Protein expressed
|
Karmellae (%)a | Relative holoprotein levelb | Relative catalytic domain levelb | |
|---|---|---|---|---|---|
| pGAL:Hmg1p | pGAPDH:Hmg1p | ||||
| RWY1494 | X | 41 ± 1 | 1.00 | ||
| RWY900 | X | Never seen | 3.60 | ||
| RWY1497 | X | X | 27 ± 1 | 1.05 | 3.30 |
a
The percent karmellae was determined from three independent experiments.
b
After adjusting for total protein loaded, the amount of holoprotein or catalytic domain was normalized to holoprotein in RWY1494.