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Karmellae biogenesis in response to β-galactosidase fusion proteins
| Protein | Karmellae (%)a
|
β-gal activityb (U/OD/min) | |
|---|---|---|---|
| 4 h | 8 h | ||
| Hmg1:β-galactosidase | 46 ± 2 | 56 ± 2 | 236 ± 12 |
| Hmg1:β-galΔ20 | 33 ± 2 | 37 ± 2 | 0.7 ± 0.4 |
The percent karmellae was determined from three independent experiments.
β-Galactosidase activity was determined from three independent experiments, running duplicate tubes for each experiment.
