Table 2.

Single nucleotide polymorphism (SNP) characteristics and sequences of TaqMan® probes designed for allele detection as supplied by the manufacturer^*

SNP	Location	NCBI reference	Probe sequence	Reporter1 Dye	Reporter 2 Dye
XO A(1936)→G	Chr2	rs17323225	GTCTCATCATTACAAATTCCAGTTA[C/T]GTTACTCCCAGGAACATCATCAGCG	VIC	FAM
XO A(2107)→G	Chr2	rs17011368	GGTCCATAAAAGGAGTTGTTCTTTA[C/T]AGCATCCTGAGGATCACAAAGAAGT	VIC	FAM
TPMT G(460)→A	Chr6	rs1800460	TCACCTGGATTGATGGCAACTAATG[T/C]TCCTCTATCCCAAATCATGTCAAAT	VIC	FAM
TPMT A(719)→G	Chr6	rs1142345	TCTCATTTACTTTTCTGTAAGTAGA[C/T]ATAACTTTTCAAAAAGACAGTCAAT	VIC	FAM
ITPA C(94)→A	Chr20	rs1127354	CGTTCAGATTCTAGGAGATAAGTTT[A/C]CATGCACTTTGGTGGCACAGAAAAT	VIC	FAM
ITPA IVS2+21A→C	Chr20	rs7270101	TGACCGTATGTCTCTGTTTTGTTTT[A/C]TTTTTAAAAGATGGTTGGATTTCTC	VIC	FAM

^*XO, xanthine oxidase; TPMT, thiopurine S-methyl transferase; ITPA, inosine triphosphatase; Chr, chromosome; NCBI, National Centre for Biotechnology Information. VIC and FAM are two different fluorescent reporter dyes available commercially from Applied Biosystems® (P/N 4316033).