Figure 2. LA stimulates cAMP production via the EP2 and EP4 receptors.
NK cells (1 × 105) were pre-incubated with 50 μM AH6809 (A) or AH23848 (B) for 30 minutes in RPMI and then stimulated with 10 μg/ml LA or 1 μM PGE2 for 1 minute. Media was removed and cells were lysed with 0.1 M HCl and boiling for 10 minutes. cAMP assays were determined by ELISA as described in materials and method. Data are graphical representatives of 3 donors in duplicate. * indicates statistically significant compared to stimulated controls, p< 0.05.