Skip to main content
. Author manuscript; available in PMC: 2009 Sep 12.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Jul 1;374(1):33–37. doi: 10.1016/j.bbrc.2008.06.082

Fig. 2. The site of interaction between TrkA/p62 and Rpt1.

Fig. 2

(A) HA-tagged TrkA along with myc-tagged WT p62, ΔN-term, or ΔUBA (as shown) was expressed in HEK 293 cells followed by NGF stimulation (50 ng/ml) for 60 min. The cells were lysed with Triton lysis buffer, and the association of TrkA with p62 and Rpt1 was determined by immunoprecipitation of the lysates with antibody to TrkA (C-14) and Western blotting with antibody to HA, myc and Rpt1. (B) The same transfected HEK lysates from above was also immunoprecipitated with anti-myc and Western blotted with HA, myc and Rpt1 antibody. Expression of myc-tagged p62 constructs is shown with an arrow.