Figure 2.

(A) Anti-coilin antibody C236 was injected into the cytoplasm of oocytes treated with CHX. After 18 h of incubation in CHX-containing OR2, Cy3-conjugated goat anti-rabbit antibody was used to detect C236 on GV spread preparations. C236 was specifically localized in the matrix of coiled bodies. (B) [³⁵S]Methionine was injected into the cytoplasm of control oocytes or oocytes treated with CHX as in A. After 24 h of incubation, cytoplasmic and nuclear proteins were separated by SDS-PAGE. The gel was stained with Coomassie blue to demonstrate that similar amounts of protein were loaded (left panel), and incorporation of [³⁵S]methionine in newly synthesized proteins was detected by autoradiography (right panel). Essentially no label occurs in the GV and cytoplasm of CHX-treated oocytes.