Figure 3.

(A) mAb 9E10 was injected into the cytoplasm of oocytes 1 h before injection of in vitro-synthesized myc-tagged coilin. After 18 h, mAb 9E10 was detected in the coiled body matrix with Cy3-conjugated goat anti-mouse antibody. The interpretation is that mAb 9E10 reacted with the myc-tagged coilin in the cytoplasm, and the two were imported together into the nucleus and were targeted to the coiled bodies. (B) Myc-tagged coilin was injected into the nucleus 3 h before mAb 9E10 was injected into the cytoplasm. Eighteen hours later, mAb 9E10 was detected in the coiled bodies. Because the antibody alone cannot enter the nucleus, the interpretation is that myc-tagged coilin shuttled to the cytoplasm and reacted with mAb 9E10, and the two entered the nucleus as an antigen–antibody complex that was targeted to the coiled bodies.