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. Author manuscript; available in PMC: 2009 Jun 26.
Published in final edited form as: Neuron. 2008 Jun 26;58(6):925–937. doi: 10.1016/j.neuron.2008.05.009

Figure 3. Changes in short-term plasticity of eEPSC by chronic TTX.

Figure 3

(A) Schematic diagram of recording and stimulation for mossy fiber EPSC.

(B) Short-term plasticity of mossy fiber synapses. EPSCs were evoked by stimulating st. lucidum (five times at 20 Hz) in the presence of gabazine (10 µM), D-AP5 (50 µM) and low dose of NBQX (0.3 µM). Mossy fiber EPSCs were measured by subtracting post-DCG-IV (2 µM) current (dotted line) from pre-DCG-IV current (thin solid line). Subtracted traces are shown as thick solid lines (subtr.). Scale bars, 50 pA and 50 ms.

(C) Group data of short-term plasticity at mossy fiber synapse shows larger depression in TTX-treated cells (n=5) than in untreated cells (n=4). *, p<0.01, Bonferroni t-test after two-way ANOVA.

(D) Diagram of recording and stimulation for recurrent synapse EPSC.

(E) EPSCs from recurrent synapses were recorded in 2 µM DCG-IV. Scale bars, 30 pA and 50 ms.

(F) Averaged short-term plasticity at recurrent synapses. Depression over stimulus is larger in TTX-treated cells (n=8) than in control (n=6). *, p<0.05, Bonferroni t-test after two-way ANOVA. For a given condition in a cell, 19–80 traces were averaged.