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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Nat Immunol. 2008 Jul 6;9(8):917–926. doi: 10.1038/ni.1632

Figure 1.

Figure 1

4-1BBL is expressed on hematopoietic stem cells and progenitors. Wild-type (WT), Tnfrsf9−/− and Tnfsf9−/− bone marrow cells were analyzed with multi-color staining. Lineage positive cells were gated out by staining with CD11b, CD3, B220, TER-119, Gr-1, and CD11c. Lineage negative cells were further characterized as described in methods. The empty histograms represent the staining of 4-1BB or 4-1BBL and the shaded histograms represent isotype controls. Mean fluorescent intensities (MFI) of isotype control and 4-1BB or 4-1BBL are indicated in the top left and right of each histogram, respectively. Data are representative of three to five independent experiments.