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. Author manuscript; available in PMC: 2008 Oct 6.
Published in final edited form as: Immunogenetics. 2008 Jul 16;60(10):575–584. doi: 10.1007/s00251-008-0315-1

Figure 2. Use of three reference strands to vary heteroduplex migration rates for improved genotyping accuracy.

Figure 2

The heteroduplexes formed when screening panel sequences are hybridized with a reference strand exhibit characteristic electrophoretic mobilities. Instances in which these heteroduplexes co-migrate make genotyping more difficult. To improve genotyping resolution and overcome genotyping errors and ambiguities, the screening panel clones were hybridized to each of three distinct reference strands: Mamu-A1*7402, Mamu-B*07, and Mafa-B*430101. This example illustrates the variation in migration rate for screening panel sequence Mamu-A1*1103 when heteroduplexed with Mamu-A1*7402 (357.8 bp), Mamu-B*07 (415.8 bp), and Mafa-B*430101 (511.3 bp). The prominent peak at 304 bp is the Mamu-B*07 reference strand homoduplex.