Figure 3. Migration values for fourteen screening panel MHC class I sequences bound to each of three fluorescently labeled reference strands.
The relative electrophoresis mobility of each screening panel MHC sequence changes dramatically depending on the reference strand used for heteroduplex formation. Migrations rates for all fourteen MHC class I sequences were determined by averaging the heteroduplex mobility from three independent instrument runs. The relative migration rates for each heteroduplex were taken into account when pooling sequences for screening panels.