Fig. 1.

CB1 and CB2 expression in human colorectal tumors. (A-B) Cnr1 mRNA expression in 19 pairs of human tumors with matched normal tissues (A) and in 10 CRC cell lines (B) was measured as described the Method. In (A), the relative expression of CB1 is the average of triplicate samples normalized against the transcript levels of h-β-actin; in (B), data are the means + SE of the relative expression from three independent experiments. (C) CB1 protein levels in these human samples were determined by western blotting. (D) Treatment with 5-aza-dC restored the expression of Cnr1 mRNA (left panel) and CB1 protein (right panel) in CRC cells lines as measured by quantitative real-time PCR and western blotting.