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. Author manuscript; available in PMC: 2008 Oct 6.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2008 Mar 3;49(6):2464–2471. doi: 10.1167/iovs.07-1639

Figure 2.

Figure 2

Geranylgeranyltransferase I expression and activity profiles in cultured TM cells. (A) Expression of GGTase-I in TM cells. RT-PCR analysis was performed using sequence-specific oligonucleotide primers corresponding to human GGTase-I and RNA extracted from human TM cells. Reactions lacking reverse transcriptase (−RT) were also set up to confirm lack of contamination of RNA with DNA. The PCR product was sequenced to confirm the identity of GGTase-I. (B) GGTase-I activity in TM cells. Soluble extracts (25 μg protein from 100,000g supernatant) of PTM cells were prepared, and GGTase-I activity was measured by quantifying the incorporation of radiolabeled isoprenoid from 3H-GGPP into Ras protein (Ras-CVLL) in the absence and presence of GGTI-DU40, as described in Materials and Methods. A representative analysis is illustrated to show the inhibition of GGTase-I activity in TM cell lysates by GGTI-DU40.