. Author manuscript; available in PMC: 2009 Sep 1.

Published in final edited form as: Bioorg Med Chem. 2008 Jul 24;16(17):8090–8097. doi: 10.1016/j.bmc.2008.07.053

Table 4.

Kinetic constants of wild-Type and CCPGCC-insertion PTPH1 and PTPα in the absence (−) and presence (+) of FlAsH when assayed with DADEpYLIPQQG

enzyme	k_cat (s^-1)	K_M (μM)	k_cat/K_M (μM^-1s^-1)

PTPH1 (−)	8.0±0.48	3.1±0.89	2.8±0.76
PTPH1 (+)	6.9±0.80	2.7±0.14	2.6±0.20

PTPH1-816 (−)	9.8±0.65	3.5±0.34	2.8±0.25
PTPH1-816 (+)	1.8±0.070	7.7±0.62	0.23±0.0093

PTPα (−)	4.2±0.27	82±9.4	0.052±0.0029
PTPα (+)	4.2±0.40	77±1.8	0.055±0.0045

PTPα-406 (−)	3.4±0.33	84±15	0.041±0.0043
PTPα-406 (+)	0.12±0.027	100±12	0.0012±0.00021