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APC were cultured in the presence of CM-Dil-labeled Pn14. (A). APC were cultured for the indicated times with 8 × 108 CFU of labeled Pn14 (800 CFU/APC). (B). APC were cultured for 240 min with the indicated concentrations of labeled Pn14. Cytochalasin D (“cyto”) was used to control for surface binding of labeled Pn14. APC were analyzed by flow cytometry. Results are representative of 2 independent experiments.
