Figure 6. Adoptive transfer of Pn14-pulsed BMM into athymic nude mice.
BMM obtained from BALB/c mice were pulsed in vitro with Pn14. BMM (i.v.) or free Pn14 (2 × 108 CFU/mouse, i.p.) were then injected into either naïve BALB/c or athymic nude (BALB/c background) mice (5 mice per group). All groups of mice were immunized i.p. 14d later with free Pn14 (2 × 108 CFU/mouse). Sera were collected on the indicated days for measurement of serum Ig titers. The data show the geometric mean +/− SEM of the individual titers. Significance (p<0.05) is indicated in the text. Representative of 2 independent experiments.