Figure 8. APC capacity of BMDC versus BMM pulsed with either free PspA or unencapsulated or encapsulated Pn for a PspA-specific T hybridoma.
BMDC and BMM obtained from BALB/c mice were cultured in the presence of the indicated concentrations of (A) PspA, (B) R6-14, or (C) R6. BALD4 (PspA-specific T hybridoma) cells were then added to these cultures, in the continued presence of the added antigen/bacteria, and cultured for 24h. Concentrations of IL-2 released by BALD4 cells in the culture SN were measured by ELISA. Representative of 2 independent experiments.