Figure 2.

Endocytosis of MAL-FLAG in COS-7 cells. COS-7 cells were transiently transfected with MAL-FLAG and, after 48 h, incubated with anti-FLAG antibody for 1 h at 4°C, and then for 15 min at either 4°C (a and b) or 37°C (c and d). Finally, the cells were fixed, permeabilized (b and d) or not (a and c) with 0.2% Triton X-100, and subjected to immunofluorescence analysis with fluorescent secondary antibodies. Bar, 10 μm.