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. 1999 Oct;10(10):3435–3447. doi: 10.1091/mbc.10.10.3435

Figure 7.

Figure 7

Comparative analysis of MAL-FLAG and transferrin internalization. COS-7 cells transiently transfected with MAL-FLAG were incubated with 0.5 mg/ml transferrin for 1 h at 37°C in the absence (a–d) or presence (e–h) of 100 μM chloroquine. Cells were then incubated with anti-FLAG antibody for 1 h at 4°C and for 5 min (a, b, e, and f) or 30 min (c, d, g, and h) at 37°C. Finally, cells were subjected to double-label immunofluorescence analysis with anti-FLAG mAb (a, c, e, and g) and polyclonal anti-transferrin antibodies (b, d, f, and h). Arrows indicate vesicles in which MAL-FLAG and transferrin colocalize. Arrowheads in d indicate vesicles with only transferrin. Bar, 10 μm.