Figure 2.

Effect of STAT3-inhibiting oligonucleotides on PANC-1 cells. PANC-1 cells were transfected with oligonucleotides at the concentrations indicated. 48 hrs later, cells were harvested, washed, and stained as in the legend to Figure 1. Percent apoptosis was quantified by subtracting the % viable population from the total. The concentration of oligonucleotide that induced 50% apoptosis (IC₅₀) is noted where appropriate. The average data from 3 experiments ± SD are shown. A: Comparison of effect of Sequences B to BB on induction of apoptosis. Sequence B has an IC₅₀ of aproximately 270 nM, while Sequence BB has an IC₅₀ calculated to be greater than 4400 nM. C: Effect of Seq D on PANC-1 cells.