TABLE 3.
Detection of human leukocyte antigen (HLA) class IB and Cw antigens by complement-mediated cytotoxicity (CMC)a
| HLA-B
|
HLA-C
|
||||||
|---|---|---|---|---|---|---|---|
| IFN-γ (−)c | IFN-γ (+) | IFN-γ (−) | IFN-γ (+) | Linkageb IFN-γ | |||
| 624-MEL | B7 | − | + | Cw7 | − | + | +++ |
| B14 | − | + | − | + | |||
| 526-MEL | B50 | − | − | − | + | NA | |
| B62 | − | + | Cw3 | − | − | ++++ | |
| 1102-MEL | B55 | + | + | Cw6 | + | + | ++++ |
| B62 | + | + | Cw3 | + | + | ++++ | |
| 697-MEL | B7 | − | + | Cw6 | − | + | ++++ |
| B15 | − | + | Cw3 | − | + | NA | |
| 938-MEL | B7 | + | + | Cw6 | + | + | ++++ |
| B8 | − | + | Cw7 | + | + | ++++ | |
| 1088-MEL | B8 | − | + | − | |||
| B44 | − | − | Cw5 | − | + | +++ | |
| 888-MEL | B52 | − | − | Cw1 | − | + | NA |
| B55 | − | + | Cw7 | + | + | NA | |
| 397-MEL | B8 | − | + | Cw7 | − | + | ++++ |
| B62 | − | + | Cw6 | − | + | NA | |
| 537-MEL | B44 | − | − | Cw5 | + | + | +++ |
| B70 | + | + | Cw1 | + | + | NA | |
| 586-MEL | B8 | − | + | Cw7 | − | + | ++++ |
| B44 | − | − | − | ||||
| 1011-MEL | B7 | − | + | Cw7 | − | + | +++ |
| B40 | − | + | Cw3 | − | + | ++++ | |
Detection of HLA class IB and Cw antigens in 11 HLA class I-positive cell lines by CMC. Positive are considered CMC scores of ≥6 (see text). The HLA-B and -C loci were paired according to their linkage disequilibrium scores as reported by Grange et al. (41).
Correlation of HLA-B vs. HLA-C locus expression in baseline conditions and after pretreatment with interferon-γ (IFN-γ): p < 0.05.
Linkage score: level of linkage disequilibrium reported to exist between HLA-B and -C shown in the same row. NA, information not available.
IFN-γ (−), detectability of HLA loci in baseline conditions; IFN-γ(+), detectability of HLA loci after pretreatment with IFN-γ (500 U/ml × 48 h).