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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Bioconjug Chem. 2008 Jul 12;19(8):1579–1587. doi: 10.1021/bc800110y

Figure 2.

Figure 2

Biosensor binding study using surface plasmon resonance. Sensorgrams obtained after injection of the CHX-A” DTPA-Z2395-C conjugate (upper panel) and parental His6-ZHER2:342 (lower panel) over a sensor chip flow-cell surface containing amine-coupled HER2-Fc fusion protein. CHX-A” DTPA-Z2395-C was injected at concentrations of 5 nM, 1.2 nM and 0.31 nM. His6-ZHER2:342 was injected at concentrations of 6 nM and 1.5 nM. Experimental curves were fitted to a 1:1 (Langmuir) binding model with correction for mass transfer effects.