Table 1. Relative lethal activity of alanine-substituted mutants in D1 of AT on SupT1 cells.

The tissue culture dose of each toxin that killed 50% of the cells (TCLD₅₀) was determined for each mutant and compared to wild type AT.

AT Mutant	Relative lethal activity (% of wild type)	AT Mutant	Relative lethal activity (% of wild type)
Loop 1 (L1)		Loop 3 (L3)
W74A	0	G289A	4
H79A	2	F290A	0
G82A	0	L291A	0
W85A	100	R292A	1
C86L	0	Y293Y	2
G87A	17	T294A	100
G88A	0	N296A	0
V94A	3	R298A	3
G95A	6	E299A	15
G97A	2	D300A	2
F98A	0	H301A	0
Loop 2 (L2)		T302A	27
R117A	13	E303A	39
Y118T	0	D304A	100
N119A	13	R305A	0
N121A	58	P306A	0
D122A	0
Y124T	0	Loop 4 (L4)
S126A	100	H329A	3
Y128T	3	Y335A	5
R129A	2	W338A	1
K131A	78	W340A	0
D132A	3
R133A	0	Helix 6 (H6)
L134A	3	W342A	0