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. Author manuscript; available in PMC: 2008 Oct 6.
Published in final edited form as: J Org Chem. 2007 May 4;72(13):4587–4595. doi: 10.1021/jo0623033

Figure 5.

Figure 5

Analysis of photolabeling of pure yPFTase with [32P]3 by SDS-PAGE. Lanes 1 and 1′contain samples of yPFTase and [32P]3b that were not irradiated. Lanes 2 and 2′ contain samples of yPFTase and [32P]3 irradiated at 350 nm. Lanes 3 and 3′ contain samples of yPFTase and [32P]3b irradiated in the presence of FPP. Lanes 1, 2 and 3 show proteins identified with Sypro®-Orange staining. Lanes 1′, 2′ and 3′ show the radiolabeled proteins identified with phosphorimaging.