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. Author manuscript; available in PMC: 2009 Jul 15.
Published in final edited form as: Clin Cancer Res. 2008 Jul 15;14(14):4672–4680. doi: 10.1158/1078-0432.CCR-08-0087

Figure 5. ΔBRIP1 exhibits defective ability to interact with BRCA1.

Figure 5

293T cells were transfected with Myc-wtBRIP1-, Myc-ΔBRIP1- or GFP-encoding plasmids. Lysates (lanes 1-3) and corresponding Myc-IPs (lanes 4-8) were analyzed by W-blotting with the indicated antibodies. BRCA1 and BARD1 were detected in Myc-wtBRIP1 complexes even when the IP was diluted up to 10 fold (lanes 4-6), whereas they were both barely detectable in undiluted Myc-ΔBRIP1 complexes (lane 7). IP from GFP-transfected cells (lane 8) served as control.